WebBIOS242, Week 3-OL. Name: Lab 3: The Gram Stain: Identify and differentiate bacteria Learning Outcomes Describe the structure of the Gram-positive and Gram-negative bacteria. Identify theoretical and technical aspects of the Gram staining procedure. Identify the most commonly made mistakes in Gram staining. Analyze the results of a Gram … Web24 B. Acid-fast Stain Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcohol (95% ethanol with 3% HCl).
Steps for Gram Staining Flashcards - Quizlet
Webgram stain is most consistent when done on young cultures of bacteria. because the gram stain is usually the first step in indentifying the bacteria. notes. old gram-positive cells … WebJan 31, 2024 · Staining procedure. cover the smear with an absorbent paper. Saturate it with carbol-fuschin and heat fix by steaming over a boiling water bath or beaker for 5-10 minutes while adding more dye to the smear. Remove the absorbent paper and decolorize it with acid-alcohol for 1 minute; rinse with tap water and tap dry. greek or latin root/affix auto
Gram Stain Flashcards Quizlet
Webwhat are the 9 steps of the procedure? 1. crystal violet- 30 secs. 2. water rinse- 5 secs. 3. gram's iodine- 1 min. 4. water rinse- 5 secs. 5. decolorize- 15-30 secs. 6. water rinse- 5 … WebStep 1: Heat the slide so that the bacterial cells are fixed to the slide Step 2: flood the slide with violet dye what is the purpose of step 2? the violet ions penetrate the thick (positive) … WebAug 1, 2024 · The Gram stain is the most widely used staining procedure in bacteriology. It is called a differential stain since it differentiates between Gram-positive and Gram-negative bacteria. Bacteria that stain purple with the Gram staining procedure are termed Gram-positive; those that stain pink are said to be Gram-negative. greek origin story summary