Ion s5tm xl
WebThe amplificates were sequenced on an Ion S5TM XL (Thermofisher, Shanghai, China) platform was used. Samples were examined for contamination with a blank PCR control (sterile water) before sequencing. The library was constructed and sequenced by Novogene Technology (Fujian, China). 2.4. WebThe library was sequenced on an Ion S5TM XL platform. Library strategy: OTHER: Library source: genomic: Library selection: other: Instrument model: Ion Torrent S5 XL : Data processing: Single-end reads was assigned to samples based on their unique barcode and truncated by cutting off the barcode and primer sequence.
Ion s5tm xl
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WebThe core gut microbiome, assessed by 16S rRNA amplicon gene sequencing on the Ion S5TM XL platform, reflects a carnivore-like signature with a dominant abundance of Firmicutes (54.96%), followed by Proteobacteria (13.98%) and Fusobacteria (12.39%), and a relatively minor contribution of Actinobacteria (10.4%) and Bacteroidetes (6.40%). WebThe Ion S5/Ion S5 XL sequencers, along with the Ion OneTouch™ 2 system, offer reproducible sequencing workflows. Delivering up to 80 million reads in a single run, the …
Web10 aug. 2024 · Then, Ion S5TM XL platform was used for library sequencing. Data Analysis The V4 region was amplified through custom barcoded primers. Raw reads were filtrated through Cutadapt software, and then the reads were compared with the Silva database for removing the chimera sequences. Finally, the clean reads were obtained. WebG@ Bð% Áÿ ÿ ü€ H FFmpeg Service01w ...
WebApplications Ion S5TMand Ion S5TMXL Systems • Simplest NGS workflow for targeted sequencing - <15mins to set up a sequencing run and <45mins total hands-on time from DNA to data with Ion Chef™ System • Fastest run time – as little as 3.5 hours from sequence to BAM files. Web14 apr. 2024 · RNA libraries were sequenced employing on 540TM Kit-OT2 on Ion S5TM XL. The transcriptomic read information had been mapped towards the annotated genome of B. bassiana BCC 2660 working with Cufflinks version 2.2.145. The genome annotation was performed employing the MAKER annotation pipeline version two.31.1046.
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Web• Ion S5™ Sequencing Reagents (Part no. A27768; 2 boxes per kit) The Ion 540™ Kit – Chef contains template preparation reagents sufficient for 8 reactions for automated … diamond card north somersetWeb• Ion S5™ Sequencing Reagents (Part no. A27768; 2 boxes per kit) The Ion 540™ Kit – Chef contains template preparation reagents sufficient for 8 reactions for automated template preparation and chip loading using the Ion Chef™ System, and reagents for sequencing using the Ion S5™ or Ion S5™ XL System. Store the kit components as diamond card kitsWebIon GeneStudio S5系列测序仪可用于DNA测序，通过检测半导体芯片核苷酸延伸过程中释放的氢离子，实现DNA分析。 该设备用于分析人类基因中不同特定区域的DNA序列。 癌 … circon newburyWeb1 dec. 2024 · The IonS5TM XL sequencing platform (Novogene company, Tianjin) was used to provide new option for microbial diversity research. Research based on semiconductor … diamond card memory matching card gameWeb20 okt. 2024 · Finally, the library was sequenced on an Ion S5TM XL platform, and 400 bp/600 bp single-end reads were generated. 2.3. Data Analysis 2.3.1. Single-End Read Quality Control The single-end reads were assigned to samples based on their unique barcode and truncated by removing the barcode and primer sequence. diamond card numberdiamond car dealershipWebThe Ion S5 XL System is simple to use with cartridge-based reagents and offers superior scalability and flexibility to support a broad range of high throughput sequencing … circonomy fraunhofer