Pcpgfree-mcs
Splet01. mar. 2024 · pCpGfree-mcs (InvivoGene, San Diego, CA, USA), which was completely devoid of CpG dinucleotides, was used as a backbone plasmid for HDI (Mitsui et al., 2009). pCpGf-HBs and pCpGf-EGFP were constructed by subcloning the hepatitis B surface antigen (HBsAg) gene and the enhanced-green fluorescent protein (EGFP) gene into the … SpletpCpGfree-mcs. Similar products. Human MCS CRISPR Knock Out 293 Cell Line. Catalog number: K1282152. Size: 1x10^6 cells/1.0ml. Price: Please ask. Human MCS CRISPR Knock Out A549 Cell Line. Catalog number: K1282153. Size: 1x10^6 cells/1.0ml. Price: Please ask. Human MCS CRISPR Knock Out HeLa Cell Line.
Pcpgfree-mcs
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SpletNucleic Acids Research Advance Access published January 20, 2015 Nucleic Acids Research, 2015 1 doi: 10.1093/nar/gkv008 A KALA-modified lipid nanoparticle containing CpG-free plasmid DNA as a potential DNA vaccine carrier for antigen presentation and as an immune-stimulative adjuvant Splet3’) was cloned into a CpG-free vector (pCpGfree-mcs Invivogen, San Diego, CA, USA) using BglII and NcoI restriction endonuclease sites (New England Biolabs Ltd., Pickering, ON, Canada). The gene was designed such that it had minimal CpG oligodeoxynucleotides and was optimized for high expression in
Splet28. feb. 2015 · To prepare pDNA encoding luciferase that is completely free from unmethylated CpG dinucleotide motifs (pCpGfree-Luc(0)), the multiple cloning site of … SpletInvivoGen pcpgfree mcs plasmid Pcpgfree Mcs Plasmid, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - …
Splet01. avg. 2014 · Hyde et al. reported that the complete deletion of the unmethylated CpG dinucleotide (CpG) motifs from the pDNA conferred a sustained gene expression with minimal inflammatory response in the lung after in … SpletTDS pCpGfree-mcs - InvivoGen. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian český русский български العربية Unknown
Splet19. avg. 2013 · To prepare pDNA encoding luciferase that is completely free from unmethylated CpG dinucleotide motifs (pCpGfree-Luc (0)), the multiple cloning site of pCpGfree-mcs (Invivogen, San Diego, CA,...
SpletpCpGfree-promoter is a reporter plasmid completely devoid of CpG dinucleotides. This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase … great shall be the peace of childrenSpletThe hHGF expression vector without any CpG, pCpGfree-hHGF was custom-made by subcloning the CpG-depleted hHGF gene into the pCpGfree-mcs vector (InvivoGen, San Diego, CA, USA). pCMV-Luc and pZsGreen1-N1 (Takara Bio Inc., Shiga, Japan) were amplified in the Escherichia coli strain DH5. pCpGfree-LacZ (InvivoGen, San Diego, CA, … floral peony desktop backgroundSpletpCpGfree-promoter: to study the effect of CpG methylation in enhancer elements. Given that many non-mammalian genes are widely used as resistance, reporter or cytotoxic genes in … floral perfume white lid squareSpletpCpGfree-mcs. Similar products. Human MCS CRISPR Knock Out 293 Cell Line. Catalog number: K1282152. Size: 1x10^6 cells/1.0ml. Price: Please ask. Human MCS CRISPR … floral perfume for herSpletpCpGfree-mcs contains an MCS featuring several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT … floral personal checksSpletExpression vector CpG-free with MCS, LacZ or mSEAP pCpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication … great shame gifSplet22. jul. 2015 · The Luc-GFP cassette was cloned by PCR cloning into pCpG F using NheI and BglII ends in order to produce the same expression cassette (CMV enhancer and EF1 promoter) in pCpG F. Vectors were prepared for all experiments using Endofree kits (Qiagen, Valencia, CA, USA). great shaman names