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WebHeat injury of L. monocytogenes was conducted at 58 degrees C for 6 min in a jacketed flask filled with tryptic soy broth. Both noninjured and heat-treated L. monocytogenes cells … WebNov 4, 2024 · Each plate yielded ~0.5 ml of harvested culture at approximately 10 log CFU/ ml. The stock cocktail was formed by combining equal volumes of all five harvested cultures and then a high-population cell suspension was prepared by combining 18 ml of the five-serotype Salmonella stock cocktail with 1,800 ml of sterile deionized water. try an app on this pc
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WebThe strain of Paenibacillus polymyxa isolated from food was grown on tryptic soy agar containing 0.6% yeast extract (TSAYE) at 30 °C for 24 h. After centrifugation, the inoculated medium was allowed to pass through a resin, and the ad‐ sorbed antimicrobial within the resin was suspended in ethanol for antimicrobial extrac‐ tion [29]. WebFor example, blood agar (GS) plates are made by enriching TSA plates with defibrinated sheep blood, and chocolate agar is made by additional cooking of GS. TSA with sheep's … WebFollowing the enrichment procedure, the inoculum was streaked on Oxford agar (OXA) containing manufacturer’s supplements and the plates were incubated at 37°C for 24–48 hrs. After 24 hrs of incubation, the growth of Listeria species on the Oxford agar plate was examined for black halo colonies, which remain the same after 48 hrs of incubation but … philip stein service center